HEXEvent - a database of Human EXon splicing Events

Home | Help | Example | Contact

Help

Genomic Location

The genomic location is specified by the chromosome, the strand and the start and end position of the region. Both position are 0-based.

e.g. chrX, - strand, 10500000-12000000

Gene List

Instead of selecting a genomic location, you can specify a list of genes, whose exons you are interested in. Gene names must be separated by commas. The server has a limit of 100 gene names at a time.

e.g. ACTB

Exon Type

If you are only interested in exons with a specific splicing behavior, you can specify this by restricting your search to certain exon types only. You can choose one or more of these categories:

Exon Type Refinement

You can refine the exon type according to your own definitions.

constitutive:
You can decide that an exon is still called constitutive, even if minor alternative splicing events are known. You can specify the percentage of ESTs showing alternative events up to which you want to call the exon constitutive.

cassette:
You can decide that you are only interested in rarely included cassette exons. In order to do so, specify up to which percentage of ESTs you allow the exon to be included.

alternative 3' splice site:
You can decide that you are only interested in exons with an alternative 3' splice site that is significantly often used. In order to do so, specify an upper bound for the usage of the major 3' splice site, which is the percentage of ESTs that show the exon with it.

alternative 5' splice site:
You can decide that you are only interested in exons with an alternative 5' splice site that is significantly often used. In order to do so, specify an upper bound for the usage of the major 5' splice site, which is the percentage of ESTs that show the exon with it.

Strict Exon Definition

When specifying the exon types you are interested in, you choose what splicing events you are interested in. In this section, you can further specify this group by either allowing your selected exons to have unselected splice events additionally or by removing all exons from your selection that also show other splice events.

A strict exon type definition selects all exons that show at least one of the exon types you selected but none of the unselected types.

A non-strict exon type definition selects all exons that show at least one of the exon types you selected and may or may not show one or more of the unselected types.

No matter whether you set your exon type definition to strict or non-strict, exons are only called constitutive, if no alternative splicing event is known (unless otherwise specified in the Exon Type Refinement, see below).